| |
Fig. 5. Expression analysis of transcription factors. (A–F′) Whole mount in situ hybridization analysis of uninjected control and xBic-C-MO1 + 2-injected embryos at stage 35. (A, A′) GATA-3; (B, B′) HNF1β; (C, C′) Lim-1; (D, D′) Pax-2; (E, E′) Pax-8; (F, F′) WT-1. Note that the expression of Lim-1 in the late distal tubule is lost upon microinjection of xBic-C-MO1 + 2 (indicated by arrowhead in C, C′). (G, H) Rescue of NBC1 expression in the late distal tubule in xBic-C-MO1 + 2-injected embryos with a single injection (2 ng) of xBic-C*, xBic-C-δKH and xBic-C-δSAM mRNA. (G) Transverse section showing expression of NBC1 in the early proximal tubule (ept) and the late distal tubule (ldt). Note that the expression of NBC1 in the late distal tubule could only be detected on the side injected with xBic-C* mRNA. (H) Quantification of the expression of NBC1 in the late distal tubule; black—bilateral expression; white—no expression; gray—unilateral expression rescued by co-injected mRNA. Each of the rescue experiments was performed at least three independent times and the graph depicts the cumulative numbers of all experiments. |
