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lhx1xenopus   

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Experiment details for lhx1

Taira M et al. (1994) Assay

Expression of the LIM class homeobox gene Xlim-1 in pronephros and CNS cell lineages of Xenopus embryos is affected by retinoic acid and exogastrulation.

Gene Clone Species Stages Anatomy
lhx1.L laevis NF stage 10.25 dorsal marginal zone
lhx1.L laevis NF stage 10.5 to NF stage 11 neuroectoderm , mesoderm , dorsal marginal zone , dorsal
lhx1.L laevis NF stage 13 neuroectoderm , intermediate mesoderm , lateral plate mesoderm
lhx1.L laevis NF stage 22 intermediate mesoderm , neural plate , pronephric mesenchyme
lhx1.L laevis NF stage 33 and 34 brain , forebrain , midbrain , hindbrain , spinal cord , [+]

  Fig. 3. Effects of RA on Xlim-1 expression as visualized by wholemount in situ hybridization. (A) Vegetal view of stage 10.25 embryo (initial gastrula). Dorsal at the top. Xlim-1 expression in the dorsal marginal zone is enhanced in the RA-treated embryo (RA; right panel) compared to control (left). (B) Stage 11 (mid-gastrula). Enhancement of Xlim-1 expression by RA is pronounced. (C) Lateral view of stage 10.5/11 embryo. Dorsal to the right. In the untreated embryo (left), dorsal mesoderm is stained but ventral mesoderm and ectoderm are not; in the RA-treated embryo, ventral mesoderm and dorsal ectoderm are also stained. The embryos were bisected for photography. (D) Dorsal view of an RA-treated embryo at stage 12 (late gastrula). Forming notochord and anterior-lateral region are stained, but presumptive somite region is not. (E) RA-treated embryo at stage 14. Assignment of staining to neural plate (white arrow) and lateral mesoderm (black arrow) was made on the basis of anterior views (see panel G). The neural staining appears to be shifted anteriorly compared to the control (Fig. 1B,D). (F) Lateral view at stage 13. Anterior (An) is left and dorsal is up. The superficial ectodermal layer is not obvious due to light printing. Filled triangles indicate the anterior edge of staining. The stained region expands more anteroventrally in the RA-treated embryo, and the ventroposterior mesoderm is also stained. (G) Anterior views with the plane of focus on the middle of embryo. Left, control embryo at stage 13; right, RA-treated embryo at stage 12 (upper and lower parts are from the same embryo at different exposures; embryo was dissected for photography). The ectoderm region between the two arrowheads is stained. (H) Dorsal view of stage 22 embryo; anterior (An) is top. The pronephros (p) in the RA-treated embryo (right) is enlarged compared with that in the control (left). (I) Ventral view of RA-treated embryo; anterior is left. Xlim-1-positive tissue connects left and right pronephroi at their anterior edge. (J) Stage 33/34. The entire hindbrain-like region in the RA-treated embryo is stained (left). In the control (right), staining is seen in the midbrain, hindbrain, spinal cord, and more weakly, in the forebrain. Apparent staining in the eyes is due to natural pigmentation. An, anterior; p, pronephros; RA, retinoic acid-treated embryos.