|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
irx3
|
|
laevis
|
NF stage 15
|
chordal neural plate
,
preplacodal ectoderm
|
irx3
|
|
laevis
|
NF stage 20
|
posterior neural tube
,
anterior placodal area
|
irx3
|
|
laevis
|
NF stage 28
to
NF stage 35 and 36
|
brain
,
midbrain
,
hindbrain
|
barhl2
|
|
laevis
|
NF stage 15
|
neural plate
|
barhl2
|
|
laevis
|
NF stage 20
|
neural tube
|
barhl2
|
|
laevis
|
NF stage 28
to
NF stage 35 and 36
|
retina
,
forebrain
,
eye
|
arx
|
|
laevis
|
NF stage 15
|
neural plate
|
arx
|
|
laevis
|
NF stage 20
|
neural tube
|
arx
|
|
laevis
|
NF stage 28
to
NF stage 35 and 36
|
forebrain
|
pax6
|
|
laevis
|
NF stage 15
|
chordal neural plate
,
anterior neural fold
|
pax6
|
|
laevis
|
NF stage 20
|
optic vesicle
,
posterior neural tube
,
neural tube
|
pax6
|
|
laevis
|
NF stage 28
to
NF stage 35 and 36
|
brain
,
forebrain
,
eye
|
otx2
|
|
laevis
|
NF stage 15
|
anterior
|
otx2
|
|
laevis
|
NF stage 20
|
optic vesicle
,
anterior
|
otx2
|
|
laevis
|
NF stage 28
to
NF stage 35 and 36
|
retina
,
forebrain
,
eye
|
lhx9
|
|
laevis
|
NF stage 20
|
optic vesicle
,
anterior neural tube
,
neural tube
|
lhx9
|
|
laevis
|
NF stage 28
to
NF stage 35 and 36
|
retina
,
brain
,
forebrain
,
midbrain
,
hindbrain
,
[+]
|
rax
|
|
laevis
|
NF stage 15
|
anterior neural fold
|
rax
|
|
laevis
|
NF stage 20
|
optic vesicle
|
rax
|
|
laevis
|
NF stage 28
to
NF stage 35 and 36
|
retina
,
eye
|
fezf2
|
|
laevis
|
NF stage 15
|
anterior neural fold
|
fezf2
|
|
laevis
|
NF stage 20
|
olfactory placode
|
fezf2
|
|
laevis
|
NF stage 28
to
NF stage 33 and 34
|
forebrain
|
|
|
Figure S2. Comparison of expression pattern of fezf2 expression relative to various
nerual markers at different stages, related to Figure 1. Anterior is to the bottom in
stage 15 and 20 embryos (frontal view), and to the left in stage 28 and 35 embryos
(lateral view). |
|
Display additional annotations [+]
|
|
Fig. 5. Fezf2 represses the activity of lhx2 and lhx9 in the forebrain. (A) ChIP-qPCR analysis of Fezf2 binding to the lhx2 promoter. Region 1 showed very high enrichment (n=3 replicates). (B,C) qPCR analysis of lhx2 and lhx9 expression in p3hGR-VP16-Fezf2-injected animal cap explants aged to stage 12 and treated with CHX alone or CHX+DEX (n=3 replicates). (D,E) qPCR analysis of lhx2 and lhx9 expression in neuralised animal cap explants aged to stage 20 (n=3 replicates). (F,G) In situ hybridisation analysis shows that mild knockdown of fezf2 leads to expansion of the lhx2 (F) and lhx9 (G) expression area. Arrowhead indicates the epithalamus; bracket indicates the ventral diencephalon. (H,I) In situ hybridisation analysis of arx (H) or ngn1 (I) in stage 28 morphants (lateral views). Arrowheads indicate arx or ngn1 expression. (J) qPCR analysis shows that lhx2 and lhx9 antagonise expression of the fezf2-induced Wnt-responsive gene xnr3 in stage 14 animal cap explants (n=3 replicates). (K) qPCR analysis shows lhx2 and lhx9 antagonise fezf2-induced ngn1 expression in stage 20 animal cap explants (n=3 replicates). In all qPCR analyses, rpl8 was used as internal control. Error bars represent s.e.m. *P<0.05, ***P<0.001; ns, not significant. |