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Fig. 6. The PI3K/Akt pathway is required for eye induction. (A) Injection of δp85 (2 ng) or dnAkt (2 ng) into dorsal animal blastomeres at the 8-cell stage blocked eye formation. (B) δp85 and dnAkt blocked the expression of rx (upper panels) and lhx2 (lower panels) at stage 14. (C) Injection of δp85 or dnAkt into A1 blastomere at the 32-cell stage impaired eye formation on the injected side (left) when embryos reached tadpole stage. Left side was injected. (D) Injection of δp85 (middle panels) or dnAkt (lower panels) into A1 blastomere at the 32-cell stage reduced the expression of rx, but not myoD, otx2, and sox3. n-β-gal was used as a lineage tracer. |
