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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
lhx2	xenopus

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Experiment details for lhx2

Zuber ME et al. (2003) Assay

Specification of the vertebrate eye by a network of eye field transcription factors.

Gene	Clone	Species	Stages	Anatomy
lhx2.S		laevis	NF stage 13	eye primordium , neural plate , anterior

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Fig. 2. Comparison of EFTF expression patterns by double whole-mount in situ hybridisation. Otx2 expression at stage 12 (A) and 13 (B). In C-I and K-T, the dark blue stain is the expression pattern of the gene named on the left, while the magenta stain is the expression pattern of the gene named on the right, at the stages shown. For example, in C, Otx2 is dark blue and Rx1 is magenta. (J) Both Emx1 and Rx1 stain dark blue. (J-L) The Rx1 (J), Pax6 (K) and Six3 (L) expression borders are indicated by a broken line. A schematic summary of the overlapping expression patterns of the eye field transcription factors at stage 12.5/13 (U) and 15 (V) is shown. Scale bars: in A, 300 μm for A-L; in M, 300 μm for M-T.

Gene	Clone	Species	Stages	Anatomy
lhx2		xenopus	NF stage 20	eye , eye primordium , optic vesicle , anterior

Fig. 3. Coordinated expression of EFTFs induces ectopic Lhx2 expression and ectopic eye-like structures outside the nervous system. (A-D) In situ hybridisation for Lhx2 expression (violet) in stage 20 embryos. (A) Uninjected embryo shows the normal expression pattern of Lhx2. (B-D) Otx2, ET, Pax6, Six3, Rx1, tll, Optx2 and β-gal RNAs were injected into one cell of two-cell stage embryos. β-gal staining (light blue) shows the injected side. Arrow indicates to ectopic Lhx2 expression (violet). (E-H) Embryos injected with Otx2, ET, Pax6, Six3, Rx1, tll and Optx2 RNAs, and grown to stage 45. Arrows indicate ectopic eyes and arrowheads point to lens. (I,J) Sections through ectopic eyes reveal the layering of ganglion (GCL), inner nuclear (INL) and outer nuclear (ONL) cell layers. (I) The retinal ganglion cells are detected using the marker, hermes (violet). Rod photoreceptors are identified in the outer nuclear layer, by the detection of opsin (green, J). Opsin also stains a rosette of cells between the GCL and the lens. Lens was detected using anti-crystalline antibodies and stains red in J. (K) Cocktail subsets reveal the relative importance of EFTFs for eye tissue induction. Animals were scored according to severity of phenotype - from ectopic pigment/eye tissue (most severe) to normal animals. When all the factors were present, most embryos developed ectopic pigment or eye tissue (Ect. Pig./Eye Tissue). When Pax6 was left out of the cocktail, for example, the frequency of ectopic pigment or eye tissue was greatly reduced and 20% of the embryos were unaffected (Normal).