|mapk1||xenopus||wound epidermis [+]|
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Experiment details for mapk1Expression of Xenopus suppressor of cytokine signaling 3 (xSOCS3) is induced by epithelial wounding.
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|Figure 4. A: Extracellular signal-regulated kinase (ERK) is activated rapidly after epithelial trauma. Embryos were wounded and allowed to heal for 2 or 10 min before fixation and immunohistochemistry for activated (bis-phosphorylated) ERK. ERK activation is evident within 2 min after trauma and is extensive after 10 min. B: Trauma-induced ERK activation is blocked by the mitogen-activated protein/ERK kinase (MEK1) inhibitor PD98059. Embryos were pretreated for 2 hr in 0.1× MMR containing either PD98059 or AG490 or SB202190 (each at 50 μM final concentration; see Table 1) before trauma. After 10 min, the embryos were fixed and immunostained for activated ERK. Inhibition of MEK1 by PD98059 resulted in a block in ERK activation after injury. Inhibition of JAK2 (AG490) or p38 (SB202190) did not affect the activation of ERK after trauma. C: Wound-induced SOCS3 expression is not inhibited by blocking MEK1 activity. Embryos were pretreated for 2 hr in 0.1× MMR containing either PD98059 or AG490 or SB202190 (each at 50 μM final concentration; see Table 1) before trauma. After 1 hr, the embryos were fixed and SOCS3 expression was assayed by in situ hybridization. Blocking MEK1 activity had no affect on the induction of SOCS3 expression at the site of epithelial wounding.Download figure to PowerPoint|