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Fig. 1. Expression of Msx1 and Msx2 in Xenopus embryos. (A) Glyoxal
RNA gel blots of 0.75 μg per lane of total RNA isolated from different
embryonic stages probed with Msx1 and Msx2. Nieuwkoop-Faber stages
are as indicated. Both transcripts appear first at early gastrula, level off at
early neurula and are maintained at roughly constant amounts through
mid tailbud stages. Ethidium bromide staining of the 18S ribosomal band
is shown to control for equal RNA loading. (B) Whole mount in situ
hybridization to late gastrula (St. 12.5) and early (St. 14) and mid-neurula
(St. 16) embryos. The expression patterns of Msx1 and Msx2 are very
similar. Expression of Slug is shown for comparison. Dorsal views, with
anterior towards the top of the figure. |