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myh6xenopus   

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Experiment details for myh6

Developmental expression and comparative genomic analysis of Xenopus cardiac myosin heavy chain genes.

Developmental expression and comparative genomic analysis of Xenopus cardiac myosin heavy chain genes.

Gene Clone Species Stages Anatomy
myh6.S laevis NF stage 37 and 38 heart , right lymph heart , left lymph heart , jaw muscle
myh6.S laevis NF stage 45 heart , cardiac mesoderm , left atrium , right atrium , cardiac ventricle , [+]
myh6.S laevis adult frog stage myocardium , left atrium , right atrium , outflow tract

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  Figure 2. In situ hybridization analysis of developmental expression of the Xenopus alpha -MHC and vMHC genes. A: Lateral view of stage 26 embryos showing alpha -MHC transcripts in the bisymmetric heart patches. B: Ventral view of embryo in A. C: Transverse section through the heart patch of stage 26 embryo. D: Lateral view of stage 30 embryo showing increased alpha -MHC expression in precardiac tissues (arrow). E: Lateral view of stage 30 embryo showing an absence of vMHC expression at this stage of development. F: Lateral view of stage 38 embryo showing alpha -MHC expression in the heart, muscles of the jaw (jm), and the lymph heart (lh). G: Transverse section through stage 36 heart shows that alpha -MHC expression is confined to the myocardium. H: Transverse section through the lymph heart (stage 38) stained for alpha -MHC. I,J: Whole-mount and section in situ hybridizations, respectively, of stage 45 heart showing that alpha -MHC is expressed in all chambers and outflow tract. K,L: Whole-mount and section in situ hybridizations, respectively, of stage 45 heart, showing restricted expression of vMHC in a ring around the outflow tract and the atria at the junction with the ventricle. M: Section in situ of adult Xenopus heart shows that alpha -MHC is expressed throughout the myocardium, including the atria (a) and outflow tract. N: Section in situ hybridization of adult Xenopus heart shows that vMHC is expressed in the myocardium of the outflow tract and ventricle but not the atria.

Gene Clone Species Stages Anatomy
myh6.S laevis adult frog stage heart , myocardium , left atrium , right atrium , outflow tract

  myh6 (myosin, heavy chain 6, cardiac muscle, alpha) gene expression in Xenopus laevis embryos, adult frog, as assayed by in situ hybridization, transverse section through heart.