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Fig. 1. A: Comparison of whole-mount in situ hybridization analysis of Xenopus MLC1v (A,J), MLC1av (D,N), and MHC alpha (G,R) RNA
expression. Left-lateral views of whole embryos are depicted (A) and also higher magnification ventral views of the heart-forming region (J).
Anterior is to the left of all images. MLC1v: stage 25 (A), stage 31 (B), stage 38 (C), stage 31 (J), stage 35 (K), stage 38 (L), stage 41 (M). MLC1av: stage
24 (D), stage 33 (E), stage 39 (F), stage 31 (N), stage 32 (O), stage 35 (P), stage 39 (Q). MHC alpha: stage 30 (G), stage 32 (H), stage 39 (I), stage 30 (R),
stage 32 (S), stage 35 (T), stage 39 (U). M: The apparent weaker MLC1v staining observed at stage 41 more likely reflects the poor tissue penetration
of antisense RNA probes that occurs at later tadpole stages. S, somites; H, heart; IH, interhyoid facial muscle; LH, lymph heart; MLC, myosin light
chain. These embryos were obtained using fertilized eggs from an albino female frog; hence, little ectodermal pigment is evident. In all figures
presented, the direction of the anteriorosterior (A-P) axis is indicated at the bottom right of key panels (A,J) by a double-headed arrow. |
