| |
Fig.6. Lurp1 morpholino disrupts myocardial morphogenesis prior to heart tube formation. (A–D) Tadpoles injected with the lurp1-MO into dorsal blastomeres, 12 ng dose, at stage 31 (A) and stage 34 (C), compared with non-injected sibling embryos (B and D). Tadpoles hybridized to mhcα (myocardium). Anterior is to left in ventral view of heart-forming regions. (E and F) Heart section of the stage 34 tadpole with dorsal blastomere morpholino and flat, bilateral myocardium (C and E), compared with control stage 34 tadpole (D and F). No counterstain. Scale bars = 100 μm. Sp, section plane; L, left; R, right; Mc, myocardium; Ec, endocardium; Lv, liver. |
