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Figure 3. Myofibrillogenesis is disrupted and sarcomere formation is delayed in dit. Confocal analysis of wild type (left panels) and dit embryos (right panels) for sarcomeric myosin heavy chain (MyHC) and Z-disc (α-actinin) immunoreactivity. (A, C) Anterior somites of stage 28 wild type embryos show sarcomeric myosin (MyHC) organized into A-bands separated by regularly spaced Z-discs (α-actinin). (B) Anterior dit somites show disorganized MyHC staining, and (D) α-actinin staining is punctate, with occasional ordered Z-bodies consistent with the appearance of incipient sarcomeres (arrowheads). (E) More posteriorly at st. 28, α-actinin staining shows wild type somites 15-16 (arrow indicates intersomitic boundary) are beginning to organize Z-discs (arrowheads), while (F) dit somites at this level show only background staining (arrow indicates intersomitic boundary). Scale bars 10 μm. |
