| |
Fig. 2. Nkx2.5 is necessary for differentiation of heart and myeloid
cells at the ventral blood island (aVBI). Embryos were
injected with Control morpholino oligos (MO) (18.4 pmol/embryo)
(b, e, h, k) or with Nkx2.5 MO (18.4 pmol/embryo) (c, f, i, l) into
the dorsal marginal zone (DMZ) region at the 4-cell stage. Intact
embryos served as a control experiment (a, d, g, j). These
embryos were allowed to develop to appropriate stages and
were processed for whole-mount in situ hybridization to examine
the expression of c/ebpa (a, b, c), spib (d, e, f), mpo (g, h, i) and
MHCa (j, k, l). Expression of myeloid cell and heart markers was
suppressed by Nkx2.5 MO injection (c, f, i, l). In contrast,
expression of these markers was not affected by Control MO (b,
e, h, k). The number in each panel indicates the total number of
samples from three independent experiments. Stained areas of
Nkx2.5 MO-injected embryos were compared to those of control
embryos and the results were classified into three categories:
“Normal” (positive staining with unreduced area), “Reduced”
(positive staining with reduced area) and “Undetected”
(no staining). Percentages of embryos with undetected, reduced
and normal expression of myeloid cell markers are shown
(m, see the details in Results). |
