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Fig. 4. XPOX2 expression within embryo explants. Left-lateral views of representative posterior-dorsal (A) and anterior-ventral (B) embryo dissections fixed at stage 21 and assayed for XPOX2 expression by in situ hybridisation. (C) Ventral view of the same fragment shown in (B). Arrows indicate the posterior limit of the XPOX2-expressing cells. Left-lateral views of posterior-dorsal (D) and anterior-ventral (E) dissections that were cultured till stage 33 and assayed as before. (F) Control stage 33 embryo with XPOX2-positive cells present in posterior and dorsal locations (arrows). The position of the myocardium (Mc) of the forming heart was also assayed by MLC2 expression (Chambers et al., 1994) in this experiment (E,F). Pigmented-wildtype embryos were used for this experiment. CG, cement gland; A, anterior; P, posterior. |
