| |
Fig. 2. Expression of nIF mRNA in the contralateral, unoperated
control eye at late stages of regeneration (A–E) and in the operated
eye 6 (F–J) and 12 (K–O) days after optic nerve crush. Neighboring
transverse sections were hybridized with digoxigenin-labeled cRNA
probes to peripherin (A,F,K), xefiltin (B,G,L), XNIF (C,H,M), NF-M
(D,I,N), and NF-L (E,J,O). A representative example of a section from
an operated eye hybridized with a sense probe to peripherin showed
no label above background (P). i, inner nuclear layer; p, retinal pigmented
epithelium (naturally pigmented and not labeled); r, retinal
ganglion cell (RGC) layer. Scale bar 100 m in P and applies to all
panels. |
