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Figure 5. Tgif represses nr5/6 expression in vegetal cells. A: Quantitative real-time polymerase chain reaction (QPCR) analysis of nr5 expression in embryos depleted of tgif1, VegT, or both. Relative expression values; samples were normalized to odc and values expressed as a percentage of uninjected control stage 10.5 embryos. tgif1-, embryos injected with 3.0 ng of tgif1-as2mp as oocytes, VegT-, embryos injected with 18.0 ng of vegt-MO as oocytes. B: Reverse transcriptase-polymerase chain reaction (RT-PCR) of mesendodermal markers in uninjected and tgif1-depleted explants. Cap, animal caps; Eq, equatorial/marginal zone region; Vg, vegetal mass/endodermal mass. Explants were cut at stage 9, and cultured to stage 11. Each sample contained five explants or two intact embryos. C-D prime : In situ hybridization of nr5/6 expression in control uninjected (B, Un) and tgif1-depleted embryos (C,D) at stage 10, obtained by fertilization of tgif1-as2mp-injected oocytes (3.0 ng) by the host-transfer method. Dorsal is toward the top. |
