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Gene/CloneSpeciesStageAnatomy ItemExperimenter
nodal6xenopus vegetal endoderm 

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Experiment details for nodal6

An essential role for transcription before the MBT in Xenopus laevis.

An essential role for transcription before the MBT in Xenopus laevis.

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Gene Clone Species Stages Anatomy
nodal6.L laevis NF stage 8 vegetal endoderm

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  Fig. 7. Nodal signaling contributes to xnr5/6 expression. (A) SB5 (200 μM) was added at the 4-cell stage and embryos were fixed for ISH at MBT (stage 8.5). Whole-embryos were photographed in vegetal views, dorsal to the top (vegetal view). Embryos bisected along the dorsal/ventral axis prior to ISH are shown with dorsal side to the right (lateral view). The frequency of the expression pattern for each group is indicated in the lower right corner. DMSO was used as a control. (B) To inhibit Nodal signaling, the dominant-negative Nodal receptor (DN-ALK4) was injected (1 ng), along with fluorescent dextran (insets, rust-colored staining), into the 2 right-hand blastomeres of 4-cell embryos. Embryos were fixed at the indicated times and analyzed by ISH. Fluorescent dextran injection alone is shown as control. The number of cells expressing xnr5 or xnr6 in injected clones was counted and significance was determined using the Kruskalallis test. (C) 8-cell embryos were injected in one animal cell with GR-tSmad2 (400 pg) and fluorescent dextran. GR-tSmad2 was activated by addition of dex for a two-hour window beginning at the indicated stages. Embryos were fixed at the end of the window and analyzed by ISH.