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Fig. 2. Expression of the X. laevis noggins at the stages 10,5–18. (A) Results of RT-PCR of the total RNA from embryos at the indicated stages with primers to noggin1 (N1), noggin2 (N2), noggin4 (N4) and EF-1-alpha (EF) as the loading control. (B-O) Results of the whole-mount in situ hybridization. The embryos are shown from the dorsal side (B, J and M), from the anterior, dorsal side upward (C, D and O), from the vegetal pole, dorsal side upward (F and G), from the right side, dorsal side upward (K, N), split sagittally, anterior to the right (E, L). (O1) Histological frontal section of the embryo shown on (O) at the level indicated by red dotted line. Abbreviations: Aem—anterior endomesoderm; Am—anterior margin of the neural plate; Anp—anterior neural plate; Ar—archenteron roof; Bl—blastopore; Dbl—dorsal blastopore lip; Ep—epidermis; Gc—gastrocoel; Nc—presumptive neural crest; Nf—neural fold; Nop—neural plate; Np/not—notoplate/notochord; Pnp—posterior neural plate; Sm—presumptive somites. |
