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Fig. 5. Overexpression of Xfz7 affects the localization of
mesodermal and neural markers. 4-cell-stage embryos were injected
at the dorsal equatorial region with 400 pg Xfz7 mRNA.
(A) Expression of Xbra at stage 10. No significant difference can be
noticed between control uninjected (top) and Xfz7-injected (bottom)
embryos. (B) Expression of Xbra in stage 12 control embryos (top) is
localised around the blastopore and in the notochordal mesoderm. In
Xfz7-injected embryos (bottom), Xbra is absent or reduced in the
notochordal mesoderm. (C) The pattern of gsc expression in stage
10.5 control embryos (top) reflects convergent extension of dorsal
mesoderm toward the blastopore. Xfz7-injected embryos (bottom) do
not display such a pattern. (D) At stage 13, gsc is located anteriorly
in control embryos (top) whereas it remains near the blastopore and
extends laterally in Xfz7-injected embryos (bottom). (E) Expression
of otx-2 in control (top) and in Xfz7-injected (bottom) embryos at
stage 10.5. A difference can be noticed as in C. (F) At stage 13,
control embryos (top) have only the anterior expression of otx-2;
Xfz7-injected embryos (bottom) show otx-2 expression around the
dorsal blastoporal lip and in the dorsal ectoderm. (G) Xnot is
expressed in the notochord in a stage 14 uninjected embryo.
(H) Expression of Xnot in the dorsal blastoporal lip in Xfz7-injected
embryos at stage 14 equivalent. (I) Expression of Sox3 is present in
the neural plate except in the midline in a stage 14 uninjected
embryo. (J) Pattern of Sox3 expression in a Xfz7-injected embryo at
stage 14 equivalent. |
