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Fig. 3. X-PAK5 K/R expression affects the localization of mesodermal markers. Four-cell stage embryos were injected either with 300 pg of gfp mRNA or with
300 pg of GFP-X-PAK5 K/R mRNA in the DMZ and fixed at the indicated time. Expression of mesodermal genes was analyzed by in situ hybridization.
Expressions in gfp control (Ctrl) and GFP-X-PAK5 K/R-injected embryos are compared in each panel. (A) Expression of Brachury (Xbra) at stage 10+. (B)
Expression of gooscoid at stage 10+. (C) Expression of Brachury (Xbra) at stage 11.5. Note that Xbra expression in GFP-X-PAK5 K/R embryos is reduced in
the notochordal mesoderm (as indicated by black arrow) indicating that in GFP-X-PAK5 K/R-injected embryos, the dorsal mesoderm failed to involute during
gastrulation. (D) Expression of Xgsc at stage 12. Black arrows indicate the two domains of Xgsc expression in GFP-X-PAK5 K/R-injected embryos. (E)
Expression of Xotx-2 at stage 12.5. Black arrows indicate Xotx-2 staining in the blastopore lip in X-PAK5-KR-expressing embryos. (F) Expression of Xnot at
early neurula stage. In gfp control (Ctrl) embryos, Xnot expression is localized in the notochordal mesoderm whereas its expression is localized in the dorsal
blastopore lip in GFP-X-PAK5 K/R-injected embryos, indicating that the notochord was not elongated in GFP-X-PAK5 K/R-injected embryos. |
