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Experiment details for not

Kodjabachian L and Lemaire P (2001) Assay

Siamois functions in the early blastula to induce Spemann's organiser.

Gene Clone Species Stages Anatomy
not.L laevis NF stage 11 dorsal marginal zone , involuted dorsal mesoderm , upper blastopore lip , axial mesoderm , dorsal , [+]

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  Fig. 3. Organiser gene expression in GR-Sia injected embryos induced at various stages. Embryos were injected ventrally with 50 pg/bl GR-Sia mRNA and 400 pg/bl LacZ RNA at the four-cell stage and DEX (10 μM) was either omitted (−DEX), or added 15 min before stage 9 (9−), or at stage 9.5. β-Galactosidase activity was revealed in red, and embryos were then processed for in situ hybridisation. For dkk1 and frzb, embryos were manually bisected prior to hybridisation. In all panels, the natural dorsal side is right. For otx2, the animal view (otx2/an) reveals the extent of the anterior neural plate. All dorsal genes examined, belonging to head and trunk classes, were robustly induced when DEX was added at stage 9−, while treatment at stage 9.5 provoked no or very weak induction.