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Experiment details for not

Yasuo H and Lemaire P (2001) Assay

Role of Goosecoid, Xnot and Wnt antagonists in the maintenance of the notochord genetic programme in Xenopus gastrulae.

Gene Clone Species Stages Anatomy
not xenopus NF stage 9 marginal zone , animal hemisphere
not xenopus NF stage 10 animal cap , marginal zone
not xenopus NF stage 11 ectoderm , involuted dorsal mesoderm , upper blastopore lip , archenteron
not xenopus NF stage 12 involuted dorsal mesoderm , upper blastopore lip , archenteron

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  Fig. 6. Comparative analysis of the expression of Xnot, gsc and frzb during the course of gastrulation. Embryos at the indicated stages were bisected along the dorsoventral axis, and processed for whole-mount in situ hybridisation analyses with the indicated probes. Dorsal is towards the right.

Gene Clone Species Stages Anatomy
not.S laevis NF stage 10.5 circumblastoporal collar
not.S laevis NF stage 13 chordal neural plate , notochord , neural groove , dorsal

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  Fig. 3. Expression of organiser genes is initiated on the ventral side when BMP signals are ectopically blocked, but is not maintained by the late gastrula stage. (A,E,I,M,Q,U) Vegetal view of uninjected mid-gastrulae (stage 11). (B,F,J,N,R,V) Vegetal view of mid-gastrulae injected ventrally with tBR RNA and lacZ RNA as lineage tracer. (C,G,K,O,S,W) Dorsal-vegetal view of uninjected late gastrulae (stage 13). (D,H,L,P,T,X) Dorsal-vegetal view of late gastrulae injected ventrally with tBR RNA. The identity of the probes used is indicated on the left of each row of panels. Note that the expression of all the genes tested is lost at the late gastrula stage, with the exception of chordin (D). β-galactosidase activity is shown in red, gene expression in purple.

Gene Clone Species Stages Anatomy
not xenopus NF stage 11.5 to NF stage 12 upper blastopore lip , axial mesoderm , dorsal
not xenopus NF stage 13 notochord , dorsal

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  Fig. 8. Regulatory interactions between Xnot, gsc and frzb during notochord formation. Embryos at the indicated stages (left) were injected with the indicated combination of factors (lower right corner of each panel) and processed for in situ hybridisation with the indicated probes (upper right corner of each panel). (A-H) Vegetal views. (I-L) Dorsal or dorso-lateral views. (M-T) Embryos were bisected along the dorsoventral axis before being processed for whole-mount in situ hybridisation. Injected cells are revealed by their β-galactosidase activity (red). Gene expression is shown in purple.