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Gene/CloneSpeciesStageAnatomy ItemExperimenter
notch1xenopus ventral animal hemisphere blastomere 

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Experiment details for notch1

Notch1 is asymmetrically distributed from the beginning of embryogenesis and controls the ventral center.

Notch1 is asymmetrically distributed from the beginning of embryogenesis and controls the ventral center.

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Gene Clone Species Stages Anatomy
notch1.L laevis NF stage 1 to NF stage 5 (16-cell) ventral animal hemisphere blastomere

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  Figure. S1. Distribution of endogenous Notch 1 and β-catenin proteins from s1 through cleavage stages in Xenopus embryos (animal views). Left and right halves of embryos were cut at the indicated Nieuwkoop and Faber stages (s) (left column, turquoise dotted boxes) and processed for whole-mount IF. The embryonic early axes were predicted according to the original pigmentation. (A,E,I,M) Bright field views. (B,F,J,N) Notch1 IF. (C,G,K,O) Total β-catenin IF. (D,H,L,P) Merged images of Notch1 and total β-catenin IF. We noted that in some early embryos, the highest levels of total β-catenin IF located relatively more dorsally (light blue asterisks) than the region were the highest levels of Notch1 IF were found (white asterisks) (see Table S2). d, dorsal; v, ventral; l, left; r, right.

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Gene Clone Species Stages Anatomy
notch1.L laevis NF stage 1 to NF stage 5 (16-cell) ventral animal hemisphere blastomere

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  Figure S2. Distribution of endogenous Notch 1 and β-catenin proteins from s1 through cleavage stages in Xenopus embryos (internal views). Left and right halves of embryos that were cut at the indicated Nieuwkoop and Faber stages (s) (left column, turquoise dotted boxes) and processed for whole-mount IF. The embryonic early axes were predicted according to the original pigmentation. (A,E,I,M) Bright field views. (B,F,J,N) Notch1 IF. (C,G,K,O) Total β-catenin IF. (D,H,L,P) Merged images of Notch1 and total β-catenin IF. We noted that in some whole-mount halves from early embryos, total β-catenin IF appeared to have a dorsal bias (light blue arrows) or its highest levels were apparently in a different place (light blue asterisk) in relation to the region were the strongest Notch1 IF was found (white asterisks) (See Table S2). d, dorsal; v, ventral; l, left; r, right; an, animal; veg, vegetal.
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