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notch1xenopus retinal ganglion cell layer 

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Experiment details for notch1

Zaghloul NA and Moody SA (2007) Assay

Alterations of rx1 and pax6 expression levels at neural plate stages differentially affect the production of retinal cell types and maintenance of retinal stem cell qualities.

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Gene Clone Species Stages Anatomy
notch1.S laevis NF stage 37 and 38 retinal ganglion cell layer

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  Fig. 5. Normal expression of stem cell (rx1, pax6, six3), neural identity (notch1, sox2) and differentiation (ngn1, p27, neuroD, Xath5) markers in control embryos. Embryos injected with β-gal mRNA were processed by in situ hybridization to determine the normal expression pattern of each marker gene. All stages 22–27 embryos are external views of whole-mount preparations [bars = 100 μm]; all stage 38 views are from whole mount preparations that were thickly sectioned to reveal internal expression patterns [bar = 20 μm]. (A) Summary of the expression domains of marker genes at stage 38 in the different layers of the retina, based on data from Perron and Harris (1999), Ohnuma et al. (1999) and our results. All genes except ngn1 are expressed in some part of the ciliary marginal zone (CMZ). Only pax6, rx1, six3 and sox2 are expressed in all zones of the CMZ. Other genes are expressed only in the zones of the CMZ (2, 3) closest to the central retina, which are thought to contain RPCs. Within the stage 38 central retina, these genes are differentially expressed in the three cellular layers (GCL, INL, ONL). L, lens. (B) rx1 is expressed throughout the optic cup (arrow). (C) At stage 38, rx1 is expressed in all zones of the CMZ and weakly in the ONL (arrow). (D) pax6 is expressed throughout the optic cup (arrow). (E) At stage 38, pax6 is expressed in the CMZ and throughout the GCL and INL. (F) six3 is expressed throughout the optic cup (arrow). (G) At stage 38, six3 is expressed throughout the CMZ, and in the GCL and INL; the faint staining in the ONL is due to the thickness of the section. (H) notch1 is expressed throughout the optic vesicle (arrow). (I) At stage 38, notch1 is expressed in zones 2 and 3 of the CMZ and throughout the GCL and INL. (J) sox2 is expressed throughout the optic vesicle (arrow). (K) At stage 38, sox2 is expressed in all zones of the CMZ, strongly in the GCL and is barely detected in the INL. (L) ngn1 is expressed in the central part of the optic cup (arrow). (M) At stage 38, ngn1 is expressed in all layers of the central retina, and is absent from the CMZ. (N) p27 is expressed broadly in the optic cup (arrow). (O) At stage 38, p27 is expressed in zones 2 and 3 of the CMZ and throughout the GCL and INL. (P) neuroD is expressed broadly in the optic cup (arrow). (Q) At stage 38, neuroD is expressed in zone 3 of the CMZ and throughout the GCL and INL. (R) Xath5 is expressed broadly in the optic cup (arrow). (S) At stage 38, Xath5 is expressed in zone 3 of the CMZ and throughout the GCL and INL.
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