|
Gene |
Clone |
Species |
Stages |
Anatomy |
notum.L
|
|
laevis
|
NF stage 3 (4-cell)
|
animal hemisphere
,
animal pole
|
notum.L
|
|
laevis
|
NF stage 6.5
|
animal hemisphere
,
animal pole
|
notum.L
|
|
laevis
|
NF stage 8
to
NF stage 10.5
|
ventral marginal zone
,
dorsal marginal zone
|
notum.L
|
|
laevis
|
NF stage 11
|
neuroectoderm
,
mesoderm
,
anterior
|
notum.L
|
|
laevis
|
NF stage 13
|
neural plate
|
notum.L
|
|
laevis
|
NF stage 15
|
epidermis
,
lateral plate mesoderm
,
non-neural ectoderm
,
pre-chordal neural plate border
|
notum.L
|
|
laevis
|
NF stage 25
|
cement gland
|
notum.L
|
|
laevis
|
NF stage 35 and 36
|
otic vesicle
,
cement gland
,
pharyngeal arch
,
hyoid arch
,
branchial arch
,
[+]
|
|
|
Figure 4. Notum Expression Patterns during Xenopus Embryogenesis
(A and B) RT-PCR revealed that Notum is maternally and zygotically expressed throughout Xenopus embryogenesis (A), and is enriched animally and dorsally at stage 10.5; animal caps, AC; dorsal marginal zone, DMZ; ventral marginal zone, VMZ; vegetal caps, VC; a pan-mesodermal marker, Xbra; a dorsal marker, Chordin; an animal and ventral marker, Msx1. (C–T) Whole mount in situ hybridization for Notum/Notum’ expression, showing lateral view at 4-cell and stage 6.5 (C and D); lateral and bisected view at stage 8.5 (E and F); bisected view at stage 9.5 (G); lateral and bisected view at stage 10.5, with dorsal on right (H and I); dorsal and bisected view at the stage 11 (J and K); with an enlarged anterior view showing stronger expression anteriorly (L); dorso-anterior (M); dorsal (N, anterior on top); and cross-section (O, dorsal on top; and P, an enlarged view) at stage 15; lateral view at the tail bud stage (Q); with an enlarged anterior view indicating expression in the cement gland (R, arrow); lateral view at the early tadpole stage (S); and with an enlarged view indicating expression in the pronephros region (T, arrowhead). The blue color at the blastocoel surface in bisections was non-specific. See also Figure S3. |
|
|
|
notum (notum, palmitoleoyl-protein carboxylesterase) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 3 (4-cell), lateral view, animal pole up.
|
|
|
|
notum (notum, palmitoleoyl-protein carboxylesterase) gene expression in bisected Xenopus laevis/ tropicalis embryo, mid-sagittal section, assayed via in situ hybridization, NF stage 10.5, dorsal left, animal pole up.
note: The light blue staining of the blastocoel surface in is non- specific. |
|
|
|
notum (notum, palmitoleoyl-protein carboxylesterase) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 15, dorsal view, anterior up.
|
|
|
|
notum (notum, palmitoleoyl-protein carboxylesterase) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 15, transverse section, mid trunk region, dorsal up. |
|
|
|
notum (notum, palmitoleoyl-protein carboxylesterase) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 22, lateral view, anterior left, dorsal up. |
|
|
|
notum (notum, palmitoleoyl-protein carboxylesterase) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35 & 36, heads region/anterior region, lateral view, anterior left, dorsal up. |