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notumxenopus   

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Experiment details for notum

Zhang X et al. (2015) Assay

Notum is required for neural and head induction via Wnt deacylation, oxidation, and inactivation.

Gene Clone Species Stages Anatomy
notum.L laevis NF stage 3 (4-cell) animal hemisphere , animal pole
notum.L laevis NF stage 6.5 animal hemisphere , animal pole
notum.L laevis NF stage 8 to NF stage 10.5 ventral marginal zone , dorsal marginal zone
notum.L laevis NF stage 11 neuroectoderm , mesoderm , anterior
notum.L laevis NF stage 13 neural plate
notum.L laevis NF stage 15 epidermis , lateral plate mesoderm , non-neural ectoderm , pre-chordal neural plate border
notum.L laevis NF stage 25 cement gland
notum.L laevis NF stage 35 and 36 otic vesicle , cement gland , pharyngeal arch , hyoid arch , branchial arch , [+]

  Figure 4. Notum Expression Patterns during Xenopus Embryogenesis (A and B) RT-PCR revealed that Notum is maternally and zygotically expressed throughout Xenopus embryogenesis (A), and is enriched animally and dorsally at stage 10.5; animal caps, AC; dorsal marginal zone, DMZ; ventral marginal zone, VMZ; vegetal caps, VC; a pan-mesodermal marker, Xbra; a dorsal marker, Chordin; an animal and ventral marker, Msx1. (C–T) Whole mount in situ hybridization for Notum/Notum’ expression, showing lateral view at 4-cell and stage 6.5 (C and D); lateral and bisected view at stage 8.5 (E and F); bisected view at stage 9.5 (G); lateral and bisected view at stage 10.5, with dorsal on right (H and I); dorsal and bisected view at the stage 11 (J and K); with an enlarged anterior view showing stronger expression anteriorly (L); dorso-anterior (M); dorsal (N, anterior on top); and cross-section (O, dorsal on top; and P, an enlarged view) at stage 15; lateral view at the tail bud stage (Q); with an enlarged anterior view indicating expression in the cement gland (R, arrow); lateral view at the early tadpole stage (S); and with an enlarged view indicating expression in the pronephros region (T, arrowhead). The blue color at the blastocoel surface in bisections was non-specific. See also Figure S3.

Gene Clone Species Stages Anatomy
notum.L laevis NF stage 3 (4-cell) animal hemisphere , animal pole

  notum (notum, palmitoleoyl-protein carboxylesterase) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 3 (4-cell), lateral view, animal pole up.

Gene Clone Species Stages Anatomy
notum.L laevis NF stage 10.5 ventral marginal zone , dorsal marginal zone

  notum (notum, palmitoleoyl-protein carboxylesterase) gene expression in bisected Xenopus laevis/ tropicalis embryo, mid-sagittal section, assayed via in situ hybridization, NF stage 10.5, dorsal left, animal pole up. note: The light blue staining of the blastocoel surface in is non- specific.

Gene Clone Species Stages Anatomy
notum.L laevis NF stage 15 non-neural ectoderm , neural plate border

  notum (notum, palmitoleoyl-protein carboxylesterase) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 15, dorsal view, anterior up.

Gene Clone Species Stages Anatomy
notum.L laevis NF stage 15 ectoderm , epidermis , gastrocoel roof plate , lateral plate mesoderm , non-neural ectoderm

  notum (notum, palmitoleoyl-protein carboxylesterase) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 15, transverse section, mid trunk region, dorsal up.

Gene Clone Species Stages Anatomy
notum.L laevis NF stage 22 epidermis , cement gland

  notum (notum, palmitoleoyl-protein carboxylesterase) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 22, lateral view, anterior left, dorsal up.

Gene Clone Species Stages Anatomy
notum.L laevis NF stage 35 and 36 otic vesicle , cement gland , pharyngeal arch , hyoid arch , branchial arch , [+]

  notum (notum, palmitoleoyl-protein carboxylesterase) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35 & 36, heads region/anterior region, lateral view, anterior left, dorsal up.