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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
nrxn3
|
|
laevis
|
unfertilized egg stage
|
oocyte
,
stage I oocyte
,
stage II oocyte
,
stage III oocyte
,
stage IV oocyte
,
[+]
|
nrxn3
|
|
laevis
|
NF stage 66
|
ovary
|
nrxn2
|
|
tropicalis
|
unfertilized egg stage
|
oocyte
,
stage I oocyte
,
stage II oocyte
,
stage III oocyte
,
stage IV oocyte
,
[+]
|
nrxn2
|
|
tropicalis
|
NF stage 66
|
ovary
|
|
|
Fig. 3. Transcripts of α-neurexins are expressed in Xenopus oocytes.
Ovarian tissue consisting of connective tissue, follicle cells and oocytes
at different stages of development was subject to wholemount in situ
hybridisation using probes specific for each of the neurexin transcripts.
(A) An nrxn I specific probe identifies transcripts equally distributed
through small, early stage oocytes (yellow arrows). At later stages, the
nrxn Iα transcript is localised to a crescent within the large oocyte (red
arrows). Transcripts were not detected in cells other than oocytes. (B,C)
Nrxn II (B) and nrxn III (C) transcripts were found in all stages of oocyte.
At the later stages, in the large oocyte, the signal is less intense probably
due to the dilution of the mRNA in the larger cytoplasmic volume. There
was no evidence for localisation of these transcripts and neither were
they detected in cells other than oocytes. Right-hand-side panels: negative
controls, hybridised with corresponding sense neurexin cRNA probes |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
nrxn3
|
|
laevis
|
NF stage 32
|
retina
,
brain
,
forebrain
,
midbrain
,
hindbrain
,
[+]
|
nrxn2
|
|
laevis
|
NF stage 32
|
retina
,
brain
,
forebrain
,
midbrain
,
hindbrain
,
[+]
|
|
|
Fig.4. Expression of α-neurexins is predominantly in neural tissue in
the tailbud embryo. (A,B) In situ hybridisation with a nrxn Iα probe
showing whole embryo (A) and an anterior neural section (B). Nrxn I is
found in cells along the length of the CNS but with a distinct gap at the
midbrain-hindbrain boundary. Sections show expression in the dorsolateral
neural tube and within the eye. (C,D) Embryos hybridised with a
nrxn IIα-specific probe show a similar pattern of staining to that seen with
nrxn I. (E,F) Embryos hybridised with a nrxn IIIα-specific probe show
much more restricted expression in the CNS and in section show ventrolateral
and eye expression in the anterior CNS (arrows). |
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nrxn1 (neurexin 1) gene expression in Xenopus laevis embryo as assayed by in situ hybridization, NF stage 32. Dorsal view: anterior left. |