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ntrk2xenopus retinal ganglion cell layer 

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Experiment details for ntrk2

The cellular patterns of BDNF and trkB expression suggest multiple roles for BDNF during Xenopus visual system development.

The cellular patterns of BDNF and trkB expression suggest multiple roles for BDNF during Xenopus visual system development.

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Gene Clone Species Stages Anatomy
ntrk2.L laevis NF stage 45 retinal ganglion cell layer

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  FIG. 4. In situ hybridization for BDNF and trkB shows the colocalization of these two messages to particular retinal areas. Fifteenmicrometer alternate cryostat sections of stage 45 Xenopus retina were hybridized with digoxygenin-labeled antisense Xenopus BDNF (A, C, E) or trkB probes (B, D, F). Specific cytoplasmic hybridization signals were localized to the ganglion cell layer (gcl) after hybridization with the BDNF probe (A, C, E), whereas specific hybridization signals were localized to both the ganglion cell layer (gcl) and innermost part of the inner nuclear layer (inl) after hybridization with the trkB probe (B, D, F). High-magnification photomicrographs of adjacent retinal sections hybridized with these two probes revealed that both BDNF (E) and trkB (F) digoxygenin-positive cell bodies colocalize to the same area in the ganglion cell layer. Arrowheads and asterisks point to two groups of cells, which are positive for the BDNF (E) and trkB (F) probes, respectively. The retinal areas outlined by the brackets in C and D are shown as high-magnification photomicrographs in E and F, respectively. Scale bar for E and F is 20 mm.
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