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Fig. 2. Expression patterns of XODC2 (A–K) and XODC1 (L–N) by whole mount in situ hybridization. (A–C) unfertilized egg and early embryos before stage 8 showing no significant signal for XODC2. (D,E) Late blastula and gastrula embryos showing strong signal. (F,G) During neurula and early tailbud stages the expression extends to both ends of the body axis and becomes gradually restricted. (H) Stage 33 embryo showing tissue-specific expression in anus, branchial arches, the surface of the lower part of cement gland, ear vesicle, forebrain, frontal gland, and inner- and outer-fin at tail tip. (I) a mid-transversal section through the head of a stage 26 embryo, showing the expression of XODC2 in stomodeal-hypophyseal anlage, and the inner layer of epidermis of the forehead area and underneath the cement gland. (J) A mid-transversal section through the head of a late tailbud (stage 30) embryo, conforming the expression in frontal gland, prosencephalon and lower surface of cement gland, as revealed in (H); moreover, it also confirms its expression in most anterior neural tube. (K) A transversal section through the tail of a stage 33 embryo confirming the expression of XODC2 in anus. (L–N) ubiquitous expression of XODC1 in early embryogenesis. In all the figures the animal pole or dorsal is up and the anterior is left. Embryo staging was according to Nieuwkoop and Faber (1956). Abbreviations: an, anus; ba, branchial arch; cg, cement gland; ev, ear vesicle; fb, forebrain; fin, fin; fg, frontal gland; ie, inner layer of epidermis; nt, neural tube; pc, prosencephalon; pr, proctodeum; sha: stomodeal-hypophyseal anlage. |
