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Fig. 7. Sox9 is not required to pattern the otic vesicle. (A) Experimental procedure. Embryos at the two-cell stage are injected in one blastomere with 1 ng of Sox9δC-GR mRNA. Embryos are subsequently incubated with dexamethasone (+Dex) at different time points during development (stage 11, 15 and 20), and fixed at stage 35 for detection of Wnt3a and Otx2 by whole-mount in situ hybridization. (B) While addition of dexamethasone at stage 11 blocks Wnt3a and Otx2 expression on the injected side, addition of dexamethasone at the stage 15 or stage 20 does not affect the regionalized expression of Wnt3a and Otx2 (arrows). RNA encoding the lineage tracer β-galactosidase was co-injected to identify the injected side (red staining), the right side for all embryos. |
