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Fig. 7.
MD3 overexpression affects the balance between cell proliferation and apoptosis in the eye field. Eye-field specification was analysed by WISH against the eye markers Rx1, Pax6, Otx2 in neurulas (stage 17; anterior view; A,A′,C,C′,E,E′) and tailbuds (stage 30; B,B′,D,D′,F,F′) injected with GFP or MD3 RNAs. Black dotted line, midline of embryo; green and red lines, enlarged expression domain of the eye markers; red asterisks, injected side of the embryo; numbers indicate the embryos analysed. Scale bar: 500 µm. Cell proliferation in the anterior region of the neurula was quantified with a BrdU staining (G) and cell death with a TUNEL assay in tailbud (H) as described in the Materials and methods; Student's t-test (P values, BrdU in G, GFP RNA=0.94, FL MD3 RNA=2.1*10−5; TUNEL in H, GFP RNA=0.8, FL MD3 RNA=2*10−3). The numbers indicate the embryos analysed (H) or the sections counted (G). NS, non significant; **P<0.01 and ***P<0.001. |
