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Figure 3. Both local (CNS) and distant (non-CNS) HCN2 channel expression restore nicotine exposure-induced mispatterning of brain markers during neural development. (A–H) Representative images of stage 25 embryos as illustrated with the angle of view marked by the black arrow. in situ hybridization with digoxigenin-labeled antisense, riboprobes were used to detect Otx2 and Xbf1 in the indicated experimental groups. Yellow arrows indicate normal expression pattern and magenta arrows indicate significantly mispatterned expression. (I,J) Quantification of in situ hybridized embryos for Otx2 (N ≥ 25 embryos for each experimental group) and Xbf1 (N ≥ 20 embryos for each experimental group) from n = 3 independent experiments pooled together. Data are represented as mean with χ2 squared test for differences in proportions, ***p < 0.001, n.s.: non-significant. |