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otx2xenopus   

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Experiment details for otx2



Knockdown of the complete Hox paralogous group 1 leads to dramatic hindbrain and neural crest defects.

Gene Clone Species Stages Anatomy
otx2.S laevis NF stage 11.5 neuroectoderm , neural plate , anterior

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  Fig. 1. Hox PG1 genes have overlapping expression domains during Xenopus laevis development. Hoxa1 (A-H), Hoxb1 (I-P) and Hoxd1 (Q-X) have overlapping expression patterns. Gastrula stage embryos (A,B,I,J,Q,R) are shown from the vegetal side, dorsal up. When compared with Otx-2 expression (* in A,I,Q), it can be seen that the Hox expression does not reach the presumptive midbrain region. Neurula embryos are shown from the dorsal side, anterior to the right (C,E,K,M,S,U) or from the anterior side, dorsal up (D,F,L,N,T,V). Later embryos are shown from the lateral side, anterior to the right (G,H,O,P,W,X). Note pharyngeal arch expression of all three PG1 genes (arrows in G,H,O,P,W,X).

Gene Clone Species Stages Anatomy
otx2.S laevis NF stage 22 forebrain , cement gland primordium , eye , optic vesicle , anterior

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  Fig. 5. Triple PG1 knockdown severely affects hindbrain patterning. Morpholinos for each of the Hox PG1 genes (A1/B1/D1MO) were co-injected into the lhs of the embryo (B,D,F,H,J). Non-injected controls are also shown (A,C,E,G,I). The hindbrain region is shown, with anterior to the top. Expression of the neural marker Nrp1 (A,B: 100%, n=11), the anterior marker Otx2 (C,D: 100%, n=10), Gbx2, which is expressed in r1 (E,F: 77%, n=13) and Krox20 (G,H: no stripes 41%; one faint stripe 35%, n=71), was analysed. Dotted line indicates the midline. In later embryos (st. 46), the neural antibody 2-G9 was used to show the morphology of the hindbrain [NIC, I; A1/B1/D1MO, injected on the left-hand side (lhs) *, J: 100%, n=5]. Rhombomere boundaries are marked.