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Fig. 3. VegT and Vg1 alter cell fates at gastrulation stages and these changes affect later neural patterning. (A) The expression domains of markers of the germ layers (top labels) during gastrulation after injection of one D1.1 blastomere with mRNAs indicated on the left. Early neural ectoderm is identified by foxD5 and otx2, non-neural ectoderm by keratin, mesoderm by Xbra and endoderm by sox17α and edd. Red cells indicate the D1.1 progeny expressing the injected mRNA, and arrows indicate regions of gene repression or ectopic expression. Frequencies of phenotypes are presented in Table 1. (B) The expression domains of pan-neural (sox3, notch1), eye field (rx1), forebrain (otx2), midbrain (en2) and hindbrain (krox20) genes during neural plate stages. White bars indicate the width of the expression domains on the injected (right) versus uninjected (left) side of the neural plate. Arrows are as above. Frequencies of phenotypes are presented in Table 1. |
