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Supplementary Fig. 4. Xotx2 and Xotx5 mRNA expression in wt and morphants. In situ hibridisation with Xotx5b and Xotx2 probes on retinal sections of either wt (Wt) or morphants (Mo1) at st. 33 and st.42. Hybridisation signal was detected by fast red and visualised under epifluorescence as described (Decembrini et al., 2006). St. 33 retinal sections were counterstained with DAPI (blue nuclei). In the pictures of st. 42 sections, fast red epifluorescence was merged to the corresponding bright field view. Notably, Xotx5b and Xotx2 mRNA detection in morphants is comparable to wt. L, lens; PE, pigmented epithelium. |
