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Fig. 2. Temporal and spatial pattern of xCyp26c expression. (A) Temporal pattern of xCyp26c expression was examined by RT-PCR analysis with cDNAs prepared from embryos at various stages. XCyp26c expression increased from the mid-late gastrula stage. This pattern is different from xCyp26a, which is ubiqui- tously expressed. (B-K) The spatial pattern of xCyp26c (B,C,F,G,J) and xCyp26a (D,E,H,I,K) expression was analyzed by whole-mount in situ hybridization. Differ- ent from xCyp26a, xCyp26c was expressed only in the presumptive anterior neural region at the early neurula stage (B,C, compared with D,E). At the mid- neurula stage, the midline of xCyp26c expression decreased (F,G). By the late neurula stage, xCyp26c expression was restricted to a set of rhombencepharic cells (J), whereas xCyp26a expression was greatly reduced (K). (L-Q) Double in situ hybridization. Brown staining indicates xCyp26c expression, while the light blue staining indicates xCyp26a (L,M), xOtx2 (N,O), and xkrox20 (P,Q) staining, respectively. Anterior view (L,N,O,P). Dorsal view (M). (Q) Magnified image of (M). r2-5 show rhombomeres 2 to 5, respectively. These data indicated that xCyp26c- positive cells were mainly identical with r2, r3, and r4. |
