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Fig. 5. Effect of retinoic acid (RA) on xCyp26c expression. (A-X)
Embryos were treated with various doses of RA at stage 8, cultured until stage 13, and then prepared for WISH analysis. No treatment (A-C). RA treatment (D-X). Concentration of RA (M) is shown on left side. Expres- sion pattern of xCyp26c (A-E), xCyp26a (I-P) and xOtx2 and xkrox20 (Q- X). Moderate doses of RA increased xCyp26c expression, but narrowed the area of expression (A-M). Over 10-6 M of RA, xCyp26c expression became restricted in the anterior neuroectoderm and underwent no further change (F-H). XCyp26a was expressed throughout the embryo (O,P), whereas xOtx2 expression completely disappeared (V-X). (Y) Real-time PCR analysis with whole embryos treated with RA. These graphs are expressed as ratio of expression level of RA-treated embryo (lane 2-7) to that of no-RA treated embryo (lane 1). |
