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Fig. 3. Expression of eye markers in X-DG morphants. Xotx2 (A, C, E, EV), Xrx1 (B, D, F, FV) and b-crystallin (G, GV) mRNA expression was detected by BMP purple
(blue staining) in Mo-X-DG-injected embryos at stage 14 (A, B), 25 (C, D) and 28 (E�GV). Nuclear beta-gal staining (red) was used to trace the side of injection. Panels
A�D show both injected and uninjected sides in frontal views. Panels E�GV show lateral view of uninjected (E, F, G) and injected (EV, FV, GV) sides, respectively. No
changes in the expression domains of Xotx2 (A) and Xrx1 (B) are detectable between the two sides at stage 14. White brackets highlight the decrease of Xotx2 (C) and
Xrx1 (D) expression in the eye of the injected sides at stage 25. Decrease is evident also at stage 28 (compare panel E to EV and F to FV); beta-crystallin expression is also
dramatically reduced in the injected side (compare panel G to GV). Mo-X-DG injection completely abolishes also Xotx2 rostral expression domain in panels E to EV. |
