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otx2xenopus   

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Experiment details for otx2

Xenopus Nbx, a novel NK-1 related gene essential for neural crest formation.

Xenopus Nbx, a novel NK-1 related gene essential for neural crest formation.

Gene Clone Species Stages Anatomy
otx2.S laevis NF stage 14 pre-chordal neural plate , anterior

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  Fig. 2. Temporal and spatial expression of Nbx during Xenopus development. (A) Temporal expression patterns of Nbx. RNA was extracted from embryos at the indicated stage of development. Nbx mRNA expression levels were measured by RT-PCR. The ubiquitous marker histone H4 served as a control. (B�I) Spatial expression patterns of Nbx. Nbx expression was analyzed by whole-mount in situ hybridization. (B) Stage 12. Black arrowheads in (B) indicate outer stripes, and white arrowheads indicate inner stripes of Nbx expression. (C) Stage 14. (D) Stage 18. (E) Stage 20. (F) Stage 25. (G) Stage 30. Arrow in (G) indicates new expression of Nbx in mesencephalon, and a transverse section of this region is shown in (I). (H) A transverse section showing the outer stripes at stage 14. (I) A transverse section of Nbx-expressing region in mesencephalon at Stage 30. (J�T) Comparison between recognized markers and Nbx expression. Whole-mount double in situ hybridizations were performed with a digoxigenin-labeled Nbx probe and a fluorescein-labeled probe for another marker. (J) Nbx (purple) and Otx2 (blue) at stage 14. (K) Nbx (purple) and Sox2 (blue) at stage 14. (L) Nbx (purple) and N-CAM (blue) at stage 16. (M, N) Nbx (purple) and Slug (blue) at stage 16. The 1 in (M) indicates the region where only Slug was expressed; the 2 indicates where Slug and Nbx were coexpressed (a transverse section of this region is shown in N); and the 3 indicates where only Nbx was expressed. (O) Nbx (purple) and Delta-1 (blue) at stage 16. (P) Nbx (purple) and -tubulin (blue) at stage 16. (Q) A schematic representation of the expression of Nbx and -tubulin. (R) An enlarged view of the outer stripe region at stage 16. Nbx (purple) and -tubulin (blue). (S, T) Nbx (purple) and - tubulin at stage 25. The transverse section of posterior region is shown in (T).