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Fig. 5. Establishment of dorsal- or ventral-specific patterns of gene expression in ext1 morphant embryos. Embryos were injected with 20 ng of EXT1 or mispair MO. In situ hybridization of vent1 (A,B); vent2 (C,D); not1 (E,F); and otx2 (G,H). Expression patterns shown are representative of at least 2/3 of embryos from 3 independent experiments. (I) Q-RT-PCR assays of organizer-specific gene expression in ext1 MO or mispair MO embryos. Relative –fold changes were evaluated as described in Fig. 3. Bars represent mean ± S.D. N=3 independent experiments. *: p < 0.05. |