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Fig. 9. Fgf and RA signaling fail to posteriorize Sall4 morphants. Whole-mount in situ hybridization of (A,E) uninjected control embryos, (B,F) embryos injected with 40 ng Sall4 MO (20 ng/blastomere at the 2-cell stage), (C,G) embryos injected with 50 pg fgf8a RNA into the right A/D blastomere, (D,H) embryos injected with 40 ng Sall4 MO (20 ng/blastomere at the 2-cell stage) and 50 pg fgf8a RNA into the right A/D blastomere, (I,K) embryos treated with 1 μM all-trans retinoic acid (ATRA) and (J,L) embryos injected with 40 ng Sall4 MO (20 ng/blastomere at the 2-cell stage) and treated with 1 μM ATRA. Expression is shown for (A-D,I,J) otx2, krox20 and hoxb9 or (E-H,K,L) sox2. Arrowheads indicate the posterior limit of otx2 expression. Dorsal views, anterior to the top. |
