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Figure 4 Effects of cnrip1 over-expression on spatial expression patterns of sox2, otx2, pax6 and rax. Whole-mount in situ hybridization
analysis was carried out on embryos uninjected or injected with 500 pg of cnrip1 mRNA into the left dorsoanimal blastomere
at the 8-cell stage. The representative results from two (B, C) or three (A, D, E) independent experiments are shown.
Anterior views with dorsal upwards. The black brackets indicate mRNA-injected left sides. The dashed lines indicate midlines of
embryos. (A) sox2 expression at stage 12.5. Injected sides of embryos (n = 24) displayed expanded expression (29%, second and
third left) or no obvious change (71%, fourth left to rightmost). (B) otx2 expression at stage 12.5. Injected sides of embryos
(n = 20) displayed expanded expression (15%, second and third left) or no obvious change (85%, fourth left to rightmost). (C)
otx2 expression at stage 24. Injected sides of embryos (n = 22) displayed expanded expression (23%, second and third left),
expanded expression in the brain but reduced expression in the eye (14%, fourth left), or no obvious change (64%, rightmost and
second right). (D) pax6 expression at stage 24. Injected sides of embryos (n = 32) displayed expanded expression (31%, second and
third left), expanded expression in the brain but reduced expression in the eye (34%, fourth left), or no obvious change (34%,
rightmost and second right). (E) rax expression at stage 24. Injected sides of embryos (n = 30) displayed expanded expression
(47%, second and third left), reduced expression (10%, fourth left), or no obvious change (43%, rightmost and second right). |
