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Fig. 1.
Tbx2 is critical for anterior neural development. (A-D) Whole-mount in situ hybridization analysis showing the expression pattern of Tbx2 at the gastrula and neurula stages. (A) Lateral view, anterior to the left. (B) Dorsal view, anterior to the top. (C, D) Anterior view, dorsal to the top. (E-L) Four-cell stage embryos were injected dorso-animally with Tbx2 (200 pg), Tbx2 MO (10 ng) and CO MO (10 ng) as indicated, cultured until stage 16–17 and then subjected to in situ hybridization against Otx2 or Krox20. LacZ RNA (100 pg) was co-injected as a lineage tracer. All embryos are shown in anterior view with dorsal to the top. Inj. denotes the injected side of embryo. (M) For axis duplication assays, embryos were injected in the ventral region with tBR (100 pg) alone or with Tbx2 (200 pg) and cultured to tadpole stages. Arrowheads and asterisks indicate eye and cement gland, respectively. (N) Quantification of axis duplication assays shown in (M). n, the total number of embryos analyzed. 2 nd axis, a partially duplicated axis; eye, a secondary eye; cg, a secondary cement gland; normal, normal embryos with single body axis.
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