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Supp F8. Orthotopic BCNE center transplantation reveals autonomous requirement for Brg1 in head formation.
(A) The experimental scheme of BCNE center transplantation (X. laevis). Note that the BCNE region is labeled with different colors in both the donor and host embryos to facilitate orthotopic grafting. (B-D) images represent dorsal views of BCNE transplanted tadpoles as merged brightfield/Alexagreen-fluorescent views. C and D show embryos with transplanted Brg1 morphant BCNE. After recording, these embryos were singly subjected to WMISH against otx2 mRNA. (B’-D’) Rows detail otx2 mRNA staining seen from left, right side and dorsal view. In wt embryos, otx2 is expressed in forebrain (including retina and olfactory epithelium [fb]), midbrain (mb) and hindbrain (hb) areas. Note the symmetric expression in the wildtype transplant, and the amorphous structure of the otx2-positiv BMO1 morphant tissue. (E) Quanitification of otx2 mRNA pattern in WT (n = 17) and BMO1 morphant (n = 24) transplants. Differences for the retina stain were significant with *, p ≤ 0,007. |
