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Fig. 3. Siamois and Twin are required for head formation. (A) Twn-MO injected embryo. Small eyes were detected in 10.3% of injected embryos (3/29). (B) Sia-MO injected embryo. Small eyes were detected in 87.5% of embryos (42/48). (C) Twn-MO and Sia-MO co-injected embryos. 80% of embryos (28/35) lost head structures. (D) Co-injection of Twn-MO and Siamois mRNA rescued the Twn-MO phenotype to normal or mild dorsalization (big eyes in 42.9% (15/35) of the embryos). (E) Sia-MO and Twin mRNA coinjected embryo. Small eyes were detected in 14.8% of embryos (4/27). (F) Control uninjected sibling embryo for (A–E). (G) Sagittal histological section of the embryo shown in C. (H) Sagittal histological section of embryo shown in F. (I) Whole-mount in situ hybridization analysis of the anterior neural marker Otx2 in stage 26 embryo co-injected with Twin-MO and Siamois-MO. (J) Otx2 expression in control embryo at stage 26. fb, forebrain; mb, midbrain; hb, hindbrain; nt, neural tube; no, notochord; he, heart. |
