Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Search Criteria
Gene/CloneSpeciesStageAnatomy ItemExperimenter
otx2xenopus   

Too many results?Too few results?

Experiment details for otx2

Rorick AM et al. (2007) Assay

PP2A:B56epsilon is required for eye induction and eye field separation.

Gene Clone Species Stages Anatomy
otx2 xenopus NF stage 13 brain

Display additional annotations [+]
  Fig. 5. The PI3K/Akt pathway is required for IGF1-induced EFTFs. (A) Western blot showing that εmor injection blocked Akt phosphorylation induced by IGF1, but had no effect on Akt phosphorylation induced by P110. In addition, εmor had no effect on IGF1-induced ERK phosphorylation. εmor (10 ng) and RNA encoding IGF1 (2 ng) or P110 (1 ng) were injected sequentially, with εmor being injected at the 1-cell stage and RNAs being injected at the 4-cell stage. Animal caps were dissected at stage 8/9 and harvested at stage 13. (B) RT-PCR results showing LY294002, a PI3K inhibitor, blocked IGF1-induced expression of rx, lhx2, pax6, and six3, without affecting IGF1-induced otx2 and sox3 expressions in animal cap assay. IGF1 was injected as described above. Caps were dissected at late blastula stage and harvested at stage 13. Some caps were exposed to LY294002 from stage 9. (C) δp85 (2 ng) and dnAkt (2 ng) blocked IGF1 (2 ng) induced rx (upper panels), but not IGF1-induced otx2 (middle panels) and sox3 (lower panels) in whole embryos. One of the dorsal animal blastomeres was injected at the 8-cell stage. The right side was injected.

Gene Clone Species Stages Anatomy
otx2 xenopus NF stage 14 brain

Display additional annotations [+]
  Fig. 6. The PI3K/Akt pathway is required for eye induction. (A) Injection of δp85 (2 ng) or dnAkt (2 ng) into dorsal animal blastomeres at the 8-cell stage blocked eye formation. (B) δp85 and dnAkt blocked the expression of rx (upper panels) and lhx2 (lower panels) at stage 14. (C) Injection of δp85 or dnAkt into A1 blastomere at the 32-cell stage impaired eye formation on the injected side (left) when embryos reached tadpole stage. Left side was injected. (D) Injection of δp85 (middle panels) or dnAkt (lower panels) into A1 blastomere at the 32-cell stage reduced the expression of rx, but not myoD, otx2, and sox3. n-β-gal was used as a lineage tracer.