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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
otx2	xenopus

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Experiment details for otx2

Zuber ME et al. (2003) Assay

Specification of the vertebrate eye by a network of eye field transcription factors.

Gene	Clone	Species	Stages	Anatomy
otx2.S		laevis	NF stage 12	pre-chordal neural plate , neural plate , anterior
otx2.S		laevis	NF stage 13	pre-chordal neural plate , neural plate , anterior

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Fig. 2. Comparison of EFTF expression patterns by double whole-mount in situ hybridisation. Otx2 expression at stage 12 (A) and 13 (B). In C-I and K-T, the dark blue stain is the expression pattern of the gene named on the left, while the magenta stain is the expression pattern of the gene named on the right, at the stages shown. For example, in C, Otx2 is dark blue and Rx1 is magenta. (J) Both Emx1 and Rx1 stain dark blue. (J-L) The Rx1 (J), Pax6 (K) and Six3 (L) expression borders are indicated by a broken line. A schematic summary of the overlapping expression patterns of the eye field transcription factors at stage 12.5/13 (U) and 15 (V) is shown. Scale bars: in A, 300 μm for A-L; in M, 300 μm for M-T.

Gene	Clone	Species	Stages	Anatomy
otx2		xenopus	NF stage 18	eye primordium , anterior

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Fig. 5. ET, Rx1 and Pax6 regulate Otx2 expression. Embryos were injected into one blastomere at the two-cell stage with RNA of the indicated gene. Whole-mount in situ hybridisation was used to detect Otx2 expression in embryos injected with 100 pg ET (B), 400 pg Rx1 (C), 200 pg Pax6 (D), 200 pg Six3 (E) or 500 pg Lhx2 (F) RNA. Embryos in A,D-F were co-injected with βgal RNA to identify the injected side. In B and C, the embryos were not stained forβ gal expression so that the repression of Otx2 could be more easily visualised. Scale bar: 300 μm. (G) Quantitation of the effect of EFTFs on Otx2 expression. Percent of embryos with an increase (↑), decrease (↓) or no change (NC) in Otx2 expression. ET induces Rx1 expression. (H,I) Rx1 injection did not effect ET expression, while ET induced Rx1 expression in Xenopus animal caps in a dose-dependent manner. Histone H4 was used as a loading control; U, uninjected; E, parallel, uninjected embryo. (J-M) Whole-mount in situ hybridisation was used to detect ET (J-K) and Rx1 (L-M) expression in stage 13 Xenopus embryos injected with 200 pg Rx1 (K) or ET (M) RNA. In (J,K), embryos were injected with βgal RNA. In L,M, GFP RNA was used to detect the injected side of the embryo. The right side is the injected side in J-M. Scale bar: 300 μm. (N) Interpretation of the results of Figs 4, 5.