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Figure 5. Excessive Doses of ONT1 Cause Dorsalization in the Embryo(A and B) The dorsalized phenotype was observed following the injection of ONT1 mRNA (200 pg; [B]).(C–F) Microinjection of ONT1 mRNA (200 pg) increased the dorsal markers Gsc ([C and D]; anterior view) and Chordin (not shown) and suppressed the ventral marker Szl ([E and F]; vegetal view).(G) Embryos were classified by morphological inspection of (H)–(L).(H–L) Stage 23 embryos received injections of BMP1 mRNA (200 pg) or ONT1 mRNA (400 pg) into the two dorsal blastomeres at the four-cell stage. Overexpression of ONT1 reversed the ventralization induced by BMP1 (I and J) but not a constitutively active form of BMPR (CA/BMPR; 6.25 pg; [K and L]).(M–O) The dorsalizing effect of ONT1 overexpression requires Chordin. In situ hybridization for the indicated markers in a control embryo (M) or in embryos given an injection of Chordin-MO (4 ng each; N) or ONT1 mRNA (200 pg) + Chordin-MO (4 ng each) (O). |
