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Fig. 2. Irx MOs causes antero-posterior neural defects. Dorsal views of Xenopus tropicalis embryos at early neurula (stg 14–15; A–R) or tadpoles (stg 42; S–X) injected with 10 ng of MOIrx1 (A, G, M, S), MOIrx2 (B, H, N, T), MOIrx3 (C, I, O, U), MOIrx4 (D, J, P, V), MOIrx5 (E, K, Q, W) or a mix of 2 ng of each MO (F, L, R, X). The MOs were co-injected with LacZ mRNA as a tracer. In all embryos, red or black arrowheads point at the injected or control side, respectively. (A–F) In Irx morphant embryos, Gbx2 is reduced and shifted caudally. (G–L). Impairment of Irx genes also caused Otx2 posterior displacement and Krox20 downregulation but does not affect Cad3 expression. (M–R) In contrast, Wnt4 expression in the spinal cord, as well as in the midbrain, is reduced. A caudal shift of the midbrain is also observed in some cases. (S–X) Later, all injected embryos show brain malformations and some of them eye defects. |
