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otx2xenopus animal hemisphere [+] 

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Experiment details for otx2

Szenker E et al. (2012) Assay

A developmental requirement for HIRA-dependent H3.3 deposition revealed at gastrulation in Xenopus.

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otx2.S image:6632834 laevis NF stage 10 animal hemisphere

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  Figure S4. Specific Effects of H3.3 MO on the Transcription of Mesoderm Genes, Related to Figure 3 (A) qRT-PCR expression profiling of MO H3.3 treated embryos. We injected CTL or H3.3 MO (9.2ng) in fertilized eggs. After incubation at 18�C to reach stage 11 (gastrula) in controls, embryos were used in each case to prepare total RNA extracts and were analyzed by qRT-PCR of indicated genes. We represent graphically in a log2 scale the fold changes between MO H3.3 and MO CTL embryos, first normalized to the housekeeping gene ODC. We classified genes according to their importance for the endoderm, mesoderm, neuroctoderm or epiderm regulatory networks. Red shows genes that are significantly downregulated in MO H3.3, while green indicates significantly overexpressed genes. In gray are genes whose expression is not affected (∗ Paired Student's t test p-value < 0.05). Plotted are means and standard deviations of 8 independent experiments performed in duplicates. (B) Spatio-temporal expression of specific markers in MO H3.3 treated embryos. We injected the indicated MO (4.6 ng) in one cell of 2-cell stage embryos and performed whole mount in situ hybridization with specific probes and stages as indicated. White arrows indicate the injected side and arrowheads the uninjected part of H3.3 morphant embryos. Scale bar: 1 mm.
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