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Fig. 7. En2 restores XTcf-4 expression in pax2/5 depleted embryos. A) 4 pmol of the indicated morpholino antisense oligonucleotides was coinjected with 200 pg of the indicated mRNA into one blastomere of two-cell stage embryos and analyzed for the expression of XTcf-4 and pax2. The asterisk marks the injected side. B) Quantification of XTcf-4 reduction in en2 and pax2/5 depleted embryos and in cross-rescue experiments. While pax5 is unable to rescue the en2 phenotype, en2 restores the XTcf-4 expression after the pax2/5 knock-down. n: number of analyzed embryos. |
