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Fig. 4. Mesoderm formation is affected by lef1 depletion. Somitic boundaries of a control X. tropicalis embryo (A). The intersomitic bound- aries (white arrow heads) are affected by lef1 depletion (B). At the ventral side boundaries are lost. Myod expression in a control X. tropicalis stage 38 embryo (C) in the ventral part of somites (black arrow-head) and migrating hypaxial musculature (open arrowheads). Myod expression is downregulated in the ventral part of somites (black arrow- head) and hypaxial musculature is absent in lef1 depleted embryos (D). Control embryos stained for pax2 expression (E) in the pronephros (black arrow), pronephric duct (open arrowhead indicates junction between pronephric duct and rectal diverticulum) and rectal diverticulum. In lef1 depleted embryos (F) the caudal part of the pronephric ducts did not grow out properly and did not join the rectal diverticula, which were rudimentary (open arrowhead indicates the posterior end of an aberrant pronephric duct). Transverse section of the pronephros (G) showing pronephric tubules in a control embryo (arrows). The pronephros of lef1 depleted embryos (H) remains a single tube (arrow), with an S-shape (cf. Fig. 4F). |
